Generator
fhlA devic

Part:BBa_K778007

Designed by: Yuka Yamazaki   Group: iGEM12_UT-Tokyo   (2012-09-23)

FhlA-generator (pLac-RBS-fhlA-d.term)

pLac-RBS-fhlA-d.term

FhlA generator (pLac)

FhlA (Part:BBa K778001) is the transcriptional activator of hycAp (Part:BBa K778000). E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

This BioBrick part can be used to overexpress FhlA with IPTG induction for enhanced hydrogen production.

UT Tokyo H2 1001+cm.png

(The modified fhlA(E363G) generator part is Part:BBa K778008.)

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the results in this wiki is older than this figure above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1457
  • 1000
    COMPATIBLE WITH RFC[1000]


Safety.png

[edit]
Categories
Parameters
None